Hepatic granulomas most commonly due to sarcoidosis and tuberculosis .
2.
The mouse model of hepatic egg granuloma of schistosoma japonicum confirmed that np30 has sensitizing effect on formation of hepatic egg granuloma , active immunity in sheeps and mice with np30 can induce good protecti on against infection with schistosoma cercariae and reduces immunopathologic damage to host , np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis
3.
It has been confirmed that np30 has sensitizing effect on formation of hepatic egg granulomas in mouse model for hepatic egg granuloma of schistosoma japonicum ; immunization with np30 in kunming mice , c57bl / 6 mice and goats obtained worm reduction of 50 . 46 % , 41 . 67 % and 42 . 78 % , respectively . np30 possesses effects of both anti - fecundity and anti - embryonation immunity on female worms of s . japonicum . moreover , np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis ( the diameter , area and volume of egg granuloma were all significantly less than those of control ; the contents of type i , iii of collagen and fibronectin were also significantly less than those of control )
4.
The target gene was then subcloned into plasmid vector and induced by iptg for its expression . after that , the recombinant protein was purified and used for the identification and characterization of its immunogenicity . the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated
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